Develop novel biophysical tools to probe ribosome specialisation.
The main goal of this theme is to develop, implement and validate novel biophysical tools to probe ribosome specialization.
This theme will focus on developing a range of new biophysical tools to probe ribosome specialisation. For example, we will develop novel tools to enhance the functional analysis of ribosomal complexes and to complement and enrich the structural characterisation delivered by Theme 3.
One of the challenges in dissecting specialised ribosomes is small sample size, given their tissue/developmental time-point specific formation. We will develop a solid-state nanopore platform to tackle this limitation by enabling ribosome analysis with single entity resolution (Raveendran et al, ACS Sensors, 2020).
To test the ability of specialised ribosomes from one species to regulate translation in another, we will develop a nanoinjection platform based on nanopipettes to introduce ribosomes into naïve cells to investigate any translation reprogramming using state-of-the-art single cell proteomics approaches.
Finally, we will generate a database platform which integrate the data types for specialised ribosomes in different species across all WPs. The database will be made freely available to the scientific community to maximise the impact of our results.
