Aim: Determine which mRNAs are translated by various specialised ribosomes.
Theme 2 will investigate the functional and mechanistic conservation of specialised ribosome components in each of our five model systems. Central to both assessing a ribosome population for specialisation and dissecting their molecular mechanism of regulation is understanding specific translational output.
To do this, this theme will engineer cell lines and organisms with affinity tagged specialized ribosome components to enable selective RiboSeq. Riboseq will determine which mRNAs are regulated by specialised ribosomes and provide information on how this regulation may be achieved. This will allow the same “type” of specialized ribosomes to be compared across multiple organisms and provide experimental evidence of functional conservation.
These analyses will also allow the identification of conserved features of mRNAs that are targeted by specialised ribosomes in each model, and inform subsequent functional testing. The role each specialised component plays in translation and its conservation will be assessed through mutational analyses, translational reporters and cross species complementation assays.
Dr Emma Thomson (she/her)
Professor Ade Whitehouse (he/him)
Professor Julie Aspden (she/her)
Dr Jo Cunningham (she/her)
Dr Nan Zhao (she/her)
Veronica Thuburn (she/her)
Dr Tessa Chan (she/her)
Dr Alison Wood (she/her)
Alex Gonçalves (he/him)
Dr Elton Vasconcelos (he/him)
Tayah Hopes (she/her)
Dr Karl Norris (he/him)